Table 2.
Yeast strains used in this study
| Strain | Abbreviation | Genotype | Source |
|---|---|---|---|
| BY4742 | Wild type | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Euroscarf |
| Y15177 | sec22Δ | MATα his3Δ1 leu2Δ0 ura3Δ0 sec22::KanMX4 | Euroscarf |
| Y21396 | BY4743 Mata/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 | Euroscarf | |
| ura3Δ0/ura3Δ0 BET1/BET1::KanMX4 | |||
| YCG7 | MATa his3Δ1 leu2Δ0 ura3Δ0 | This study | |
| bet1::KanMX4 sec22::LEU2 +pCG36-BET1 | |||
| YCG8 | sec22(Q)/ | MATa his3Δ1 leu2Δ0 ura3Δ0 | This study |
| bet1(R) | bet1::KanMX4 sec22::LEU2 +pCG64-bet1S86R-sec22R157Q | ||
| Y21581 | BY4743 Mata/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 | Euroscarf | |
| ura3Δ0/ura3Δ0 SED5/SED5::KanMX4 | |||
| YCG15 | sed5(R) | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | This study |
| sed5::KanMX4 +pCG32-sed5Q283R | |||
| YCG74 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | This study | |
| sed5::KanMX4 +pCG51-sed5Q283R | |||
| YCG2 | MATα his3Δ1 leu2Δ0 ura3Δ0 | This study | |
| sed5::KanMX4 sec22::LEU2 +pCG34-SED5 | |||
| YCG3 | sec22(Q)/ | MATα his3Δ1 leu2Δ0 ura3Δ0 | This study |
| sed5(R) | sed5::KanMX4 sec22::LEU2 +pCG62-sed5Q283R-sec22R157Q | ||
| Y22689 | BY4743 Mata/α, his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2D0/LYS2 MET15/met15Δ0 | Euroscarf | |
| ura3Δ0/ura3Δ0 BOS1/bos1::KanMX4 | |||
| YCG9 | MATa met15Δ0 ura3Δ0 bos1::KanMX4 | This study | |
| +pCG8-bos1Q186R +pUA4-sec22R157Q | |||
| YCG38 | sec22(Q)/ | MATα his3Δ1 ura3Δ0 leu2Δ0 bos1::KanMX4 sec22::KanMX4 | This study |
| bos1(R) | +pCG8-bos1Q186R +pUA4-sec22R157Q | ||
| YCG84 | MATα lys2Δ0 sed5::KanMX4 sec22::LEU2 bet1::KanMX4 +pCG64-bet1S86R- | This study | |
| sec22R157Q +pCG34-SED5 | |||
| YCG103 | bet1(R)/ | MATα lys2Δ0 sed5::KanMX4 sec22::LEU2 bet1::KanMX4 | This study |
| sed5(R) | +pCG64-bet1S86R-sec22R157Q +pCG32-sed5Q283R |