Table 3.
Summary of phenotypes caused by `0' layer mutations tested in this study
| ER—Golgi SNAREs
|
||||||||
|---|---|---|---|---|---|---|---|---|
| Sec22 | Sed5 | Bos1 | Bet1 | Other SNAREs | Growth | Transport of CPY (37°C) | Morphology (electron microscopy) | |
| R | Q | Q | S | wt | Vacuolar form | Wild type (reference) | ||
| Q | Q | Q | S | Ts- | ER—cis-Golgi form | — | ||
| Q | Q+R | Q | S | wt | Vacuolar form | — | ||
| Q | Q | Q+R | S | Ts- | ER—cis-Golgi form | — | ||
| Q | Q | Q | S+R | wt | Vacuolar form | — | ||
| Q | Q | Q | S+Q | Ts- | ER—cis-Golgi form | — | ||
| 2R:2Q | ||||||||
| R | R | Q | S | Ts-* | — | Accumulation of ER-like membranes; fragmented vacuoles | ||
| R | R | Q | S | + ykt6(Q) | Ts- | — | Accumulation of ER-like membranes | |
| R | R | Q | S+Q | Ts-* | — | — | ||
| R | R | Q | S+Q | + ykt6(Q) | Ts- | — | — | |
| R | Q | R | S | lethal | — | — | ||
| R+Q | Q | R | S | Ts- | — | — | ||
| R+Q | Q | R | S+Q | Ts- | — | — | ||
| R | Q | Q | R | wt | — | — | ||
| Q | R | Q | R | Ts-* | — | — | ||
| Q | R | Q | R | + ykt6(Q) | wt | — | — | |
| Swaps | ||||||||
| Q | R | Q | S | Ts-* | ER and Golgi form | Fragmented vacuoles | ||
| Q | R | Q | S | + ykt6(Q) | wt | Vacuolar form | Resembling wild type | |
| Q | Q | R | S | Ts- | ER and vacuolar form | — | ||
| Q | Q | Q | R | wt | Vacuolar form | — | ||
Ts-, restrictive temperature 37°C; Ts-*, restrictive temperature 33°C; wt, wild-type growth.