Plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection

. Author manuscript; available in PMC: 2025 Jan 10.

Published in final edited form as: Cell Host Microbe. 2023 Dec 15;32(1):93–105.e6. doi: 10.1016/j.chom.2023.11.020

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-TET8	Hailing Jin (He et al.¹⁵)	N/A
Mouse monoclonal anti-GFP	Sigma-Aldrich	Cat#11814460001
Rabbit polyclonal anti-mCherry	Abcam	Ca#ab167453
Rabbit polyclonal anti-PR1	Agrisera	Cat# AS10 687
Rabbit immunoglobin G	Thermo Fisher	Ca# 02-6102
Bacterial and virus strains
One Shot^™ TOP10 Chemically Competent E. coli	Thermo Fisher	Cat#C404003
E. coli TET8-mCherry	Hailing Jin (Cai et al.¹⁰)	N/A
E. coli ANN1-mCherry	Hailing Jin (He et al.¹⁵)	N/A
E. coli SAG21-YFP	This paper	N/A
E. coli APS1-YFP	This paper	N/A
E. coli mSAG21-YFP	This paper	N/A
E. coli mAPS1-YFP	This paper	N/A
E. coli SAG21-3WJ-4xBro	This paper	N/A
E. coli APS1-3WJ-4xBro	This paper	N/A
E. coli PRXIIC-3WJ-4xBro	This paper	N/A
E. coli HEL-3WJ-4xBro	This paper	N/A
E. coli OEP6-3WJ-4xBro	This paper	N/A
Biological samples
Plant Extracellular vesicles	Arabidopsis. thaliana	N/A
Fungal hyphae	Botrytis. cinerea	N/A
Nicotiana benthamiana leaves	Nicotiana benthamiana	N/A


Chemicals, peptides, and recombinant proteins
Puromycin	Sigma	CAS#58-58-2
DFHBI-1	Sigma	CAS#1241390-29-3
FM4-64	Thermo Fisher	Cat#T13320
uranyl acetate	LADD	N/A
SAG21-YFP	This paper	N/A
APS1-YFP	This paper	N/A
Critical commercial assays
NEB Next Poly(A) mRNA Magnetic Isolation Module kit	NEB	#E7490
NEBNext^® Ultra^™ Directional RNA Library Prep Kit	NEB	#E7420
MITO-ID Membrane potential detection kit	Enzo	EZN-51018


Deposited data
RNA-seq data	This paper	GEO: GSE197077




Experimental models: Cell lines





Experimental models: Organisms/strains
Arabidopsis SAG21-YFP	This paper	N/A
Arabidopsis APS1-YFP	This paper	N/A
Arabidopsis mSAG21-YFP	This paper	N/A
Arabidopsis mAPS1-YFP	This paper	N/A
Arabidopsis SAG21-3WJ-4xBro	This paper	N/A
Arabidopsis APS1-3WJ-4xBro	This paper	N/A
Arabidopsis PRXIIC-3WJ-4xBro	This paper	N/A
Arabidopsis HEL-3WJ-4xBro	This paper	N/A
Arabidopsis OEP6-3WJ-4xBro	This paper	N/A
Arabidopsis ANN1-mCherry	Hailing Jin (He et al.¹⁵)	N/A
Arabidopsis T-DNA line SALK_099663	TAIR	SALK_099663
Arabidopsis T-DNA line SALK_046518	TAIR	SALK_046518
Botrytis SAG21-YFP	This paper	N/A
Botrytis APS1-YFP	This paper	N/A
Botrytis YFP	This paper	N/A
Botrytis mSAG21-YFP	This paper	N/A
Botrytis mAPS1-YFP	This paper	N/A
Oligonucleotides
See Table S5 for primers	This paper	N/A




Recombinant DNA
SAG21-YFP	This paper	N/A
APS1-YFP	This paper	N/A
mSAG21-YFP	This paper	N/A
mAPS1-YFP	This paper	N/A
SAG21-3WJ-4xBro	This paper	N/A
APS1-3WJ-4xBro	This paper	N/A
PRXIIC-3WJ-4xBro	This paper	N/A
HEL-3WJ-4xBro	This paper	N/A
OEP6-3WJ-4xBro	This paper	N/A
ANN1-mCherry	Hailing Jin (He et al.¹⁵)	N/A
Software and algorithms

Image J	National Institutes of Health	https://imagej.net/ij/
FastQC	Simon Andrews	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
MultiQC	Ewels et al.⁷²	https://multiqc.info/
STAR (v. 2.7.5a)	Dobin et al.⁷³	http://star.mit.edu/
Rsubread package	Liao et al.⁷⁴	https://bioconductor.org/packages/release/bioc/html/Rsubread.html
edgeR package in R	Robinson et al.⁷⁵	https://bioconductor.org/packages/release/bioc/html/edgeR.html
signalP (v. 5.0b)	Almagro Armenteros et al.⁷⁷	https://services.healthtech.dtu.dk/services/SignalP-5.0/9-Downloads.php
Other