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. Author manuscript; available in PMC: 2024 Feb 16.
Published in final edited form as: Cell Rep. 2023 Nov 4;42(11):113389. doi: 10.1016/j.celrep.2023.113389

Figure 4. APT-mT interactomes and localization.

Figure 4.

(A) Volcano plot of APT1-mT prey relative to parental prey based on LFQ intensities.

(B) Volcano plot of APT2-mT prey relative to parental prey based on LFQ intensities.

Red proteins in (A) and (B) have increased labeling by the APT-mT construct based on a Perseus analysis with S0 = 2. Cyan- or blue-labeled proteins in (A) and (B) are proteins of interest for future study.

(C) Principal-component analysis of LFQ intensities from mT MS data.

(D) Confocal microscopy of mT-expressing HepG2 cells. V5: tag for mT constructs. Tomm20: mitochondrial marker. Hoechst: nuclear marker. Scale bars: 10 μm (top) and 2 μm (bottom).

See also Figure S2 and Table S3.