. Author manuscript; available in PMC: 2025 Jan 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Dec 6;1232:123954. doi: 10.1016/j.jchromb.2023.123954

Table 3:

Accuracy and extraction efficiency of analyte standards in solvent matrix^a.

Analyte^a	Measured (ng/mL)	%CV^b	RE^c (%)	EE^d (%)
25OHD₃-S
1 ng/mL	0.90 ± 0.12	13	−12	66 ± 16
5 ng/mL	4.84 ± 0.4	8.2	−3.2	67 ± 12
20 ng/mL	20.6 ± 1.5	7.5	3	77 ± 15
VitD₃-S
1 ng/mL	0.95 ± 0.05	5.7	−5	47 ± 15
5 ng/mL	4.78 ± 0.47	9.8	−4.4	48 ± 12
20 ng/mL	21.35 ± 0.93	4.3	7	59 ± 19

Statistical data are mean ± SD, n=4.

Analytes were suspended in 60% MeOH for measurement.

Inter-assay coefficient of variation (CV), n=4.

Relative error (RE) is [(measured - theoretical) /theoretical]*100%.

Extraction efficiency (EE) is [(with extraction) /( without extraction)]*100%.