Table 4:
Recovery of Analytes in 250 uL Breastmilk
| Analysis | Theoreticala ng/mL | Measured ng/mL (n) |
Intra-assay %CVb (n) |
Inter-assay %CVb (n) |
REc (%) | EEd(%) | MEe (%) |
|---|---|---|---|---|---|---|---|
| 25OHD3-S | |||||||
| Spike, solvent | 4.7 | 4.68 ± 0.34 (6) | 3 (3) | 7.2 (6) | −0.4 | 67 ± 11 | - |
| Baseline Breastmilk | - | <0.2 | <LOQ | <LOQ | - | - | - |
| Spiked Breastmilk | 4.84 | 6.81 ± 0.75 (15) | 6.4 (9) | 11.0 (15) | 41 | - | 139 ± 17 |
| VitD3-S | |||||||
| Spike, solvent | 4.7 | 5.35 ± 0.3 (6) | 5.0 (3) | 5.4 (6) | 14 | 59 ± 8 | - |
| Baseline Breastmilk | - | 1.70 ± 0.23 (15) | 8.8 (9) | 13.5 (15) | - | - | - |
| Spiked Breastmilk | 6.40 | 6.87 ± 0.92 (15) | 9.3 (9) | 13.5 (15) | 7.3 | - | 94 ± 20 |
| IS [1,25(OH)2D3-G] | |||||||
| Solvent | - | - | 15.7 (n=4) | 20.4 (12) | - | 60 ± 6 | - |
| Breastmilk | - | - | 7.5 (n=9) | 8.4 (17) | - | - | 169 ± 22 |
Statistical data are means ± SD. Replicates are derived from a common pooled sample.
a
Theoretical concentration is baseline measurement + 4.7 ng/mL of analyte.
b
Coefficient of variation (CV).
c
Relative error (RE) is [(measured - theoretical) /theoretical]*100%.
d
Extraction efficiency (EE) is [(with extraction) /(without extraction)]*100%.
e
Matrix effect (ME) is ([spike]breastmilk / [spike]solvent)*100%.