. Author manuscript; available in PMC: 2025 Jan 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Dec 6;1232:123954. doi: 10.1016/j.jchromb.2023.123954

Table 5:

Liquid-liquid extraction of spiked breastmilk

Extraction Method	Aqueous Extract ng/mL	RE^e (%)	Organic Extract ng/mL	RE^e (%)
Method 1^a
25OHD₃-S	3.35	−7.4	Not Detected	−100
VitD₃-S	6.3	9.6	0.44	−92
Percent^d	97%	-	3%	-
Method 2^b
25OHD₃-S	10.2	182	0.13	−96
VitD₃-S	6.5	13	0.53	−91
Percent^d	96%	-	4%	-
Method 3^c
25OHD₃-S	3.6	−0.5	<0.2	−99
VitD₃-S	5.4	−6	0.14	−98
Percent^d	97%	-	3%	-

Separation by hexane:methanol:water.

Acetonitrile precipitation and separation by hexane:methylene chloride.

Methanol precipitation and separation by hexane:methylene chloride.

Percent abundance is averaged from both analytes.

Relative error (RE) is [(measured - theoretical) /theoretical]*100% with theoretical values of 3.62 ng/mL 25OHD₃-S and 5.75 ng/mL VitD₃-S