Figure 4.

Pharmacological agents that disrupt actin or rafts inhibit BCR internalization. DKOS cells were grown with (CHC⁻) or without (CHC⁺) doxycycline. (A) Cells were treated for 30 min with 2 μM latrunculin B (Lat), stock dissolved in DMSO, or the equivalent concentration of DMSO alone. (B) Cells were treated for 30 min with 25 μg/ml nystatin (Nys) in 0.05% methanol (Meth) or 0.05% Meth alone. BCR internalization was monitored after each treatment as described in Figure 2.