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. 2005 May;16(5):2363–2371. doi: 10.1091/mbc.E04-10-0878

Figure 5.

Figure 5.

Effects of FXYD3 on the transport and enzymatic properties of Na,K-ATPase. (A) Three days after injection of rat Na,K-ATPase α1 (10 ng) and β1 (1 ng) cRNAs in the absence or presence of FXYD3 (2 ng) cRNA, K+-activation constants (K½ K+) of the Na,K-ATPase were determined in the presence of 90 mM external Na+. Open circles, Na,K-ATPase alone; closed circles, Na,K-ATPase plus FXYD3. Shown are means ± SE of 10 oocytes from two different batches. Phosphate-buffered saline <0.05 at all membrane potentials. (B) Two days after injection of rat α1 (10 ng) and β1 (1 ng) cRNAs in the absence or presence of FXYD3 (2 ng) cRNA, the epithelial Na+ channel subunit cRNAs (α, β, and γ, 0.3 ng each) were injected. After overnight incubation, Na+ activation constants (K½ Na+) were determined at–50 mV. Shown are means ± SE from 11 oocytes from three different batches. *p < 0.01. For electrophysiological measurements, endogenous, oocyte Na,K-ATPase was inhibited by the presence of 1 μM ouabain.