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DNCs, LNCs, and fibrillar caps are distinct compartments. Confocal images of (A) untreated: PSF is nucleoplasmic, whereas fibrillarin was in the nucleoli; treated: PSF and fibrillarin relocalized in the two different nucleolar caps. This was true also for (B) PSF and UBF, (C) PSF and gar1. Bar, 10 μm. (D) Fibrillarin is found in phase light nucleolar caps (LNCs), whereas UBF, representing the transcriptional machinery, is found in the adjacent fibrillar caps.
