Figure 3.

Immunoprecipitation of Bax oligomers causes depletion of MAC activity. (A) Total mitochondrial lysates and fractions containing oligomeric and monomeric Bax of HeLa cells were immunoprecipitated with anti-Bax antibodies (Bax antibody) or total rabbit IgG (control antibody). The pellets containing the immunoprecipitated proteins (P) and their supernatant (S) were subjected to SDS-PAGE, and the presence of Bax was assessed by Western blot. (B) The supernatants from the immunoprecipitation assays described above and corresponding to immunoprecipitates with anti-Bax antibodies (Bax antibody, closed) or with total rabbit IgG (Control antibody, open) were dialyzed and reconstituted in proteoliposomes. MAC detection frequency was determined by patch clamping. n = 20–23 independent patches/condition. Other conditions were as described in Figure 2.