Skip to main content
. 2005 May;16(5):2424–2432. doi: 10.1091/mbc.E04-12-1111

Figure 6.

Figure 6.

An increase in outer membrane permeability occurs in mitochondria of staurosporine-treated parental and Bax-deficient cells but not Bax/Bak deficient cells. (A) Western blots show expression of Bax and Bak in three MEF cell lines: lane 1, parental Bax+/+Bak+/+; lane 2, single Bax knockout (KO) Bax-/-Bak+/+; and lane 3, double KO (DKO) Bax-/- Bak-/-. Total membrane extracts containing 20 μg of protein were probed for Bax and Bak, respectively. The same results were obtained with whole cell extracts (our unpublished data). (B) Mitochondria were isolated from the indicated cell lines that were and were not treated with staurosporine for 16 h. The mean conductance of the outer membrane was measured by patch clamping isolated mitochondria (n = 20–23 patches per condition). (C) Cytochrome c release was assayed by ELISA in the supernatants after permeabilization of the cells with digitonin as described in Materials and Methods. When indicated, alamethicin (80 μg/ml) was added during digitonin treatment as a positive control for cytochrome c release (Polster et al., 2001).