TABLE 1.
Immune responses of mice 11 weeks after immunization by the i.v. route with different numbers of viable BCG
| Treatment | Mouse | No. of cytokine-producing cellsa/106 spleen cells
|
Serum antibody titerb
|
||
|---|---|---|---|---|---|
| IFN-γ | IL-4 | IgG1 | IgG2a | ||
| Saline only | C1 | 4 | 2 | 200 | — |
| C2 | 2 | 6 | — | 400 | |
| C3 | 2 | 0 | — | 200 | |
| C4 | 4 | 8 | — | 200 | |
| BCG CFU | |||||
| ∼2 × 102 (low dose) | L1 | 292 | 4 | — | — |
| L2 | 98 | 0 | — | — | |
| L3 | 101 | 6 | 100 | 100 | |
| L4 | 188 | 0 | 200 | — | |
| L5 | 98 | 14 | 200 | 100 | |
| ∼2 × 106 (high dose) | H1 | 246 | 222 | 8 × 104 | 3.2 × 105 |
| H2 | 212 | 278 | 4 × 104 | 1.6 × 105 | |
| H3 | 332 | 302 | 8 × 104 | 3.2 × 105 | |
| H4 | 326 | 300 | 4 × 104 | 3.2 × 105 | |
| H5 | 398 | 370 | 8 × 104 | 3.2 × 105 | |
a
As determined by antigen-dependent ELISPOT assay.
b
Defined as the last dilution to give a positive result when tested by ELISA. All sera from mice before immunization had titers of <100 (—).