Table 1.
Yeast strains used in this study
Strain | Genotype | Source |
---|---|---|
BJ2168 | MATaleu2 trp1 ura3 pep4-3 prb1-1122 prc1-407 | Yeast Genetic Stock Center |
YYK200 | BJ2168 atg17Δ::kanMX | This study |
YYK416 | BJ2168 ATG1::HA-ATG1 atg17Δ::kanMX | This study |
YYK422 | BJ2168 ATG1::HA-ATG1 ATG17::FLAG-ATG17 | This study |
YYK426 | BJ2168 ATG1::HA-ATG1 ATG17::FLAG-ATG17 atg13Δ::kanMX | This study |
YYK424 | BJ2168 atg1Δ::LEU2 ATG17::FLAG-ATG17 | This study |
YYK467 | BJ2168 ATG1::HA-ATG1 ATG17::FLAG-atg17C24R | This study |
SEY6210 | MATα his3 leu2 lys2 trp1 ura3 suc2 | Robinson et al. (1988) |
KVY55 | SEY6210 pho8::pho8Δ60 | Kirisako et al. (1999) |
YTS007 | KVY55 atg9Δ::kanMX | This study |
YYK382 | KVY55 atg17Δ::kanMX | This study |
YYK475 | KVY55 atg11Δ::URA3 | This study |
YYK381 | SEY6210 pep4Δ::LEU2 atg17Δ::kanMX | This study |
KA311A | MATahis3 leu2 trp1 ura3 | Irie et al., (1993) |
YYK36 | KA311A atg1Δ::LEU2 | Kamada et al. (2000) |
YYK111 | KA311A atg17Δ::HIS3 | Kamada et al. (2000) |
PJ69–4A | MATahis3-200 leu2-3, 112 trp1-901 ura3-52 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ | James et al. (1996) |
TN124 | MATaura3 trp1 pho8::pho8Δ60 pho13::LEU2 | Noda et al. (1995) |
TN124–1C | TN124 atg1-1 | This study |
TN125 | MATaade2 his3 leu2 lys2 trp1 ura3 pho8::pho8Δ60 | Noda et al. (1995) |
TN126 | TN125 atg1Δ::LEU2 | Kamada et al. (2000) |