Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat anti-human C1s | Complement Technology | Cat# A204 |
| Rabbit Anti-Human C5b-9 | Bioss Antibodies | Cat# bs-2673R |
| HRP-conjugated Goat Anti-Mouse IgG | SouthernBiotech | Cat# 1030-05 |
| HRP-conjugated Goat Anti-Mouse IgM | SouthernBiotech | Cat# 1021-05 |
| HRP-conjugated Goat Anti-Human IgG | SouthernBiotech | Cat# 2040-05 |
| HRP-conjugated Goat Anti-Human IgM | SouthernBiotech | Cat# 2020-05 |
| Alexa Fluor 488 conjugated Goat Anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat# A-11034 |
| Biotin anti-human IgG | BioLegend | Cat# 410718 |
| Biotin anti-human IgM | BioLegend | Cat# 314504 |
| Bacterial and Virus Strains | ||
| Citrobacter rodentium (DBS100 strain) | ATCC | ATCC# 51459 |
| Escherichia coli (Nissle 1917 strain) | Danino et al.71 | N/A |
| Staphylococcus aureus (SH1000 strain) | Archer et al.72 | N/A |
| Staphylococcus lentus (B3 strain) | This manuscript | N/A |
| Biological Samples | ||
| Human breast milk | Mother’s Milk Bank | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Hoechst 33342 | Thermo Fisher Scientific | Cat# H1399 |
| FM5-95 | Thermo Fisher Scientific | Cat# T23360 |
| DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) | Sigma-Aldrich | Cat# D8417 |
| DTT (Dithiothreitol) | Sigma-Aldrich | Cat# D9779 |
| FITC-dextran | Sigma-Aldrich | Cat# FD4 |
| Oxytocin | Sigma-Aldrich | Cat# O4375 |
| Ponceau S solution | Sigma-Aldrich | Cat# P7170 |
| NuPAGE LDS Sample Buffer | Thermo Fisher Scientific | Cat# NP0007 |
| DiBAC4(3) [Bis-(1,3-Dibutylbarbituric Acid) Trimethine Oxonol] | Cayman Chemical | Cat# 33924 |
| CCCP (Carbonyl cyanide m-chlorophenyl hydrazone) | Cayman Chemical | Cat# 25458 |
| Recombinant Human CD59 Protein | Sino Biological | Cat# 12474-H08H |
| Uranyl Acetate | Electron Microscopy Sciences | Cat# 22400 |
| 2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer | Electron Microscopy Sciences | Cat# 16537-15 |
| Neomycin sulfate | Santa Cruz Biotechnology | Cat# sc-3573 |
| Vancomycin Hydrochloride | Santa Cruz Biotechnology | Cat# sc-204938A |
| Cefoxitin | Sagent Pharmaceuticals | NDC 25021-109-10 |
| Sterilizable Fenbendazole Diet | Envigo | Cat# TD.01432 |
| Phenol:Chloroform:Isoamyl Alcohol (25:24:1) | Sigma-Aldrich | Cat# P2069 |
| Tryptic Soy Broth | Millipore | Cat# 22092 |
| MacConkey agar | Criterion | Cat# C6131 |
| Lennox L Broth | Research Products International | Cat# L24066 |
| Lennox L Agar | Research Products International | Cat# L24030 |
| Bovine Serum Albumin | Research Products International | Cat# A30075 |
| Trypsin Protease | Thermo Fisher Scientific | Cat# 90057 |
| Critical Commercial Assays | ||
| NucleoSpin Tissue Kit | Macherey-Nagel | Cat# 740952.50 |
| Mouse Immunoglobulin Isotyping ELISA kit | Thermo Fisher Scientific | Cat# 88-50630-88 |
| BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23225 |
| TruSeq Nano DNA Library Prep Kit | Illumina | Cat# 20015965 |
| GeneJET Gel Extraction Kit | Thermo Fisher Scientific | Cat# K0691 |
| Ion Plus Fragment Library Kit | Thermo Fisher Scientific | Cat# 4471252 |
| Streptavidin Magnetic Beads | Thermo Fisher Scientific | Cat# 88816 |
| BeadBug prefilled tubes with 0.1mm Silica glass beads | Sigma-Aldrich | Cat# Z763721 |
| Milk Bacterial DNA isolation Kit | Norgen Bioteck | Cat# 21550 |
| PureLink Microbiome DNA Purification Kit | Thermo Fisher Scientific | Cat# A29789 |
| Phusion High-Fidelity PCR Master Mix | New England Biolabs | Cat# M0531S |
| Deposited Data | ||
| 16S rRNA gene profiling data | NCBI | Accession: BioProject PRJNA796457 |
| S. lentus B3 whole genome sequencing data | NCBI | Accession: BioProject PRJNA796456 |
| S. lentus H29 | GenBank | GenBank accessions: CP059679 |
| S. lentus NCTC12102 | GenBank | GenBank accessions: UHDR01000002 |
| S. aureus MW2 | NCBI | NCBI Reference Sequence: NC_003923 |
| S. epidermids RP62A | NCBI | NCBI Reference Sequence: NC_002976 |
| S. haemolyticus JCSC1435 | NCBI | NCBI Reference Sequence: NC_007168 |
| S. saprophyticus ATCC15305 | NCBI | NCBI Reference Sequence: NC_007350 |
| SwissProt 2020 Mus musculus database | UniProtKB | https://www.uniprot.org |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57Bl/6J mice | Jackson Laboratory | Strain# 000664 |
| Mouse: C1qc−/−: C57BL/6NJ-C1qcem1(IMPC)J/J | Jackson Laboratory | Strain# 029409 |
| Mouse: C3−/−: B6.129S4-C3tm1Crr/J | Jackson Laboratory | Strain# 029661 |
| Mouse: μMT−/−: B6.129S2-Ighmtm1Cgn/J | Jackson Laboratory | Strain# 002288 |
| Software and Algorithms | ||
| GraphPad Prism 9.4.0 | GraphPad | https://www.graphpad.com/ |
| FlowJo 10.9.0 | BD | https://www.flowjo.com/solutions/flowjo |
| Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
| Adobe Photoshop | Adobe | https://www.adobe.com/products/photoshop.html |
| Proteome Discoverer v2.4 | Thermo Fisher Scientific | https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis/proteome-discoverer-software.html |
| Mascot v.2.6.2 | Matrix Science | https://www.matrixscience.com/mascot_support_v2_6.html |
| Scaffold 4 | Proteome Software | https://www.proteomesoftware.com |
| ShinyGO v0.741 | South Dakota State University | http://bioinformatics.sdstate.edu/go/ |
| FV31S-SW_V2.1 | Olympus | https://www.olympus-lifescience.com/en/downloads/detail-iframe/?0[downloads][id]=847252002 |
| Fiji | Image J | https://imagej.net/software/fiji/downloads |
| Trimmomatic software V0.32 | Bolger et al.73 | http://www.usadellab.org/cms/index.php?page=trimmomatic |
| QIIME2 | QIIME 2 development team74 | https://library.qiime2.org/about/ |