Figure 7.

Overexpressing GDP-rab6a inhibits Golgi scattering in response to nocodazole. HeLa cells stably expressing GalNAcT2-GFP were microinjected with plasmids encoding GDP-rab6a at a stock concentration of 100 ng/μl. After 6-h incubation at 37°C cells were treated with 10 μM nocodazole at 37°C for 1 h. Then, cells were fixed and antibody stained for rab6 and GM130. The localizations of GM130 (A) and GalNAcT2 (B) are shown. Arrowheads (A) indicate microinjected cells. In the presence of nocodazole, a number of scattered fluorescence structures positive for GalNAcT2 and GM130 were observed in noninjected cells but not in cells injected with rab6a-GDP. Arrow (A), GM130-positive tubular extension. Bar, 10 μm.