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. Author manuscript; available in PMC: 2025 Feb 1.
Published in final edited form as: Trends Genet. 2023 Nov 20;40(2):118–133. doi: 10.1016/j.tig.2023.10.012

Figure 2. Lentiviral vectors for single-cell pooled CRISPR screens.

Figure 2.

Direct Pol3 capture vectors can be used to directly recover the functional gRNA in a pooled CRISPR screen. Direct Pol2 capture allows for the direct recovery of the gRNA sequence with a polyA tail matching the functional gRNA. Indirect Pol2 capture allows for the inference of gRNA sequence by capturing a barcoded capture sequence with a polyA tail.