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. Author manuscript; available in PMC: 2024 Feb 16.
Published in final edited form as: Cell Rep. 2023 Nov 18;42(12):113462. doi: 10.1016/j.celrep.2023.113462

Figure 5. Enzymatic function of Gαo variants.

Figure 5.

(A) GTP binding and hydrolysis (left) by purified wild-type Gαo equilibrated in BODIPY-GTP, without (middle) and with (right) normalization.

(B) BODIPY-GTP binding and hydrolysis by purified group 1 variants, without (top) and with (bottom) detectable catalytic activity.

(C) BODIPY-GTP binding and hydrolysis by purified group 2 variants, with wild-type-like (top) and with slow (bottom) rates of hydrolysis.

(D) BODIPY-GTP binding and hydrolysis by purified group 3 variants.

(E) BODIPY-GTP binding and hydrolysis by purified group 4 variants. Data are representative of six measurements from two independent purifications. See Data S3.