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. 2023 Nov 23;13(2):166–176. doi: 10.1093/stcltm/szad079

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© The Author(s) 2023. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Intrasplenic implantation of hESC-derived cholangiocyte-like cells and analysis after implantation. (A) The procedure of introducing cholangiocyte-like cell cluster into the mice spleen. Black arrow: cholangiocyte-like cell cluster. (B) HE staining of the spleen section obtained from sham mice and mice receiving implantation of cholangiocyte-like cell cluster (cholangiocyte-c group) 6 weeks after implantation. (C) Immunostaining of human-specific CK19 in the spleen section 6 weeks after implantation. The shame-operated mice were used as control.