Figure 11.

Effect of PL and PN supplementation on the E. coli BW25113 wild type and pdxI knock out strains. Growth curves of wild type and pdxI knock out strains were obtained by measuring the optical density at 600 nm. The E. coli strains were grown in M9 minimal medium containing 0.4% glucose as sole carbon source supplemented with PL or PN, at the indicated concentrations. Each curve represents the average ± SEM of 3 independent experiments each performed in triplicate. The continuous line results from fitting to a four-parameters growth equation. A) comparison of growth curves obtained with wild type and pdxI strains in M9 minimal medium supplemented with either 0.1 mM PL or PN. The effect of higher concentrations of either PN or PL on both strains are shown in panels B (wild type) and C (pdxI).