Figure 4.

T cell development is abnormal in CD3η-expressing retrogenic mice. (A) Total thymocyte numbers in retrogenic Rag1^−/− mice reconstituted with CD247^−/− BM expressing CD3ζ or CD3η. (B) Left, representative flow cytometric analysis of CD4 and CD8 expression in the thymus from retrogenic mice expressing CD3ζ or CD3η. Cells were first gated on expression of the relevant reporter in each case; right, quantification of the percentages of thymic populations in CD3ζ or CD3η mice. (C) The numbers of CD4 and CD8 SP thymocytes in retrogenic CD3ζ or CD3η mice. (D) Representative flow cytometric analysis and quantification of the percentages of CD44 and CD25 expression in DN thymocytes from retrogenic mice expressing CD3ζ or CD3η. (E) Representative flow plot and the summary of TCRβ MFI in DP, CD4 and CD8 SP thymocytes in retrogenic CD3ζ or CD3η mice. (F) Quantification of CD5 MFI in DP thymocytes in retrogenic CD3ζ or CD3η mice. Results shown are the mean ± SEM of 5–9 mice per group and each dot represents a single retrogenic mouse. Statistical analysis was determined by unpaired Student’s t-test (A,F) or two-way ANOVA (B-E) with Sidak post-test correction. *p<0.05, **p<0.01, ****p<0.0001; ns: not significant.