REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
LanthaScreen Tb-anti-GST Antibody	Thermo Fisher Scientific	Cat#PV3550
Bacterial and virus strains
TurboCells BL21 (DE3) Competent E. coli	Genlantis	Cat#C302020
Chemicals, peptides, and recombinant proteins
Dimethyl sulfoxide	Thermo Fisher Scientific	Cat#BP231-100
T0901317	Cayman Chemical	Cat#71810
Rosiglitazone	MedChemExpress	Cat#HY-17386
GW0742	MedChemExpress	Cat#HY-13928
SJPYT-299	This paper	N/A
SJPYT-300	This paper	N/A
SJPYT-301	This paper	N/A
SJPYT-302	Lin et al.27	N/A
SJPYT-303	This paper	N/A
SJPYT-304	This paper	N/A
SJPYT-305	This paper	N/A
SJPYT-315	Lin et al.27	N/A
SJPYT-316	Lin et al.27	N/A
SJPYT-317	Lin et al.27	N/A
SJPYT-318	This paper	N/A
SJPYT-319	Lin et al.27	N/A
SJPYT-320	This paper	N/A
Dulbecco's Modified Eagle's Medium (DMEM)	ATCC	Cat#30-2002
RPMI-1640 Medium	ATCC	Cat#30-2001
Leibovitz's L-15 Medium	ATCC	Cat#30-2008
DMEM, high glucose, HEPES, no phenol red	Thermo Fisher Scientific	Cat#21063029
Fetal bovine serum (FBS), Canadian Origin	Cytiva	Cat#SH30396.03
HyClone Charcoal/Dextran treated FBS, US Origin	Cytiva	Cat#SH30068.03
Hygromycin B (50 mg/mL)	Thermo Fisher Scientific	Cat#10687010
Zeocin Selection Reagent	Thermo Fisher Scientific	Cat#R25001
Geneticin Selective Antibiotic (G418 Sulfate) (50 mg/mL)	Thermo Fisher Scientific	Cat#10131027
Matrigel Growth Factor Reduced Basement Membrane Matrix	Corning	Cat#356230
Sodium Pyruvate (100 mM)	Thermo Fisher Scientific	Cat#11360070
MEM Non-Essential Amino Acids Solution (100X)	Thermo Fisher Scientific	Cat#11140050
NEBuilder HiFi DNA Assembly Master Mix	New England Biolabs	Cat#E2621S
KpnI-HF	New England Biolabs	Cat#R3142S
FAM-SRC1-B Peptide: FAM-(PEG)5-CPSSHSSLTERHKILHRLLQEGSPS-NH2	Lin et al.46	N/A
Magnesium chloride, 1M aq. soln., sterile-filtered	Thermo Fisher Scientific	Cat#J62411AD
Bovine Serum Albumin	Sigma-Aldrich	Cat#A3294
Ethylenediamine Tetraacetic Acid (EDTA)	Thermo Fisher Scientific	E478-500
Isopropanol	Thermo Fisher Scientific	Cat#A416-4
Ethylene glycol	Sigma-Aldrich	Cat#102466-500ML
Pluronic F-127, 0.2 μm filtered (10% Solution in Water)	Thermo Fisher Scientific	Cat#P6866
Lysozyme, Egg White	Gold Biotechnology	Cat#12650-88-3
Terrific Broth, Modified, Granulated, 5 Kilograms	Research Products International	Cat#T15100-5000.0
D-(+)-Glucose	Sigma-Aldrich	Cat#G8270
SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free	Sigma-Aldrich	Cat#S8830
IPTG	Gold Biotechnology	Cat#I2481C100
Ampicillin (Sodium), USP Grade	Gold Biotechnology	Cat#A-301-100
DTT (Dithiothreitol) (> 99% pure) Protease free	Gold Biotechnology	Cat#DTT100
Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane]	Research Products International	Cat#T60040-1000.0
Sodium Chloride	Research Products International	Cat#S23020-500.0
Glycerol, 99+%, extra pure	Thermo Fisher Scientific	Cat#158920025
Imidazole, 99%	Thermo Fisher Scientific	Cat#122025000
HisTrap FF	Cytiva	Cat#17525501
HiLoad 26/600 Superdex 200 pg	Cytiva	Cat#28989336
FuGENE HD Transfection Reagent	Promega	Cat#E2311
ProxiPlate-384 Plus F, Black 384-shallow well Microplate	PerkinElmer	Cat#6008260
CulturPlate-384, White Opaque 384-well Microplate, Sterile and Tissue Culture Treated	PerkinElmer	Cat#6007680
PXR (SXR) Ligand Binding Domain Protein	Thermo Fisher Scientific	Cat#PV4841
LXR-a Recombinant Human Protein, Ligand Binding Domain, GST-Tagged	Thermo Fisher Scientific	Cat#PV4657
LXR-b Recombinant Human Protein, Ligand Binding Domain, GST-Tagged	Thermo Fisher Scientific	Cat#PV4660
His-tagged PXR LBD	This paper	N/A
Unlabeled SRC-1 Peptide: N-CPSSHSSLTERHKILHRLLQEGSPS-C	This paper	N/A
Critical commercial assays
CellTiter-Glo Luminescent Cell Viability Assay	Promega	Cat#G7572
steadylite plus Reporter Gene Assay System	PerkinElmer	Cat#6066751
LiveBLAzer FRET-B/G Loading Kit with CCF4-AM	Thermo Fisher Scientific	Cat#K1096
Maxwell 16 LEV simplyRNA Tissue Kit	Promega	Cat#AS1280
SuperScript VILO cDNA Synthesis Kit	Thermo Fisher Scientific	Cat#11754050
TaqMan Fast Advanced Master Mix	Thermo Fisher Scientific	Cat#4444557
TaqMan gene expression assay for CYP3A4	Thermo Fisher Scientific	Cat#Hs00604506_m1
TaqMan gene expression assay for ABCA1	Thermo Fisher Scientific	Cat#Hs01059118_m1
TaqMan gene expression assay for GAPDH	Thermo Fisher Scientific	Cat#Hs03929097_g1
TaqMan gene expression assay for RNA18S	Thermo Fisher Scientific	Cat#Hs03928990_g1
Deposited data
Crystal structure of PXR LBD bound to SJPYT-318	This paper	PDB ID 8SZV
Experimental models: Cell lines
293T/17 Cells	ATCC	Cat#CRL-11268
GeneBLAzer LXRα-UAS-bla HEK 293T Cells	Thermo Fisher Scientific	Cat#K1692
GeneBLAzer LXRβ-UAS-bla HEK 293T Cells	Thermo Fisher Scientific	Cat#K1699
GeneBLAzer PPARδ-UAS-bla HEK 293T Cells	Thermo Fisher Scientific	Cat#K1690
GeneBLAzer PPARγ-UAS-bla HEK 293H Cells	Thermo Fisher Scientific	Cat#K1701
SNU-C4 Cells	Korean Cell Line Bank	Cat#0000C4
SNU81 Cells	Korean Cell Line Bank	Cat#00081
SNU719 Cells	Korean Cell Line Bank	Cat#00719
LS1034 Cells	ATCC	Cat#CRL-2158
SNU620 Cells	Korean Cell Line Bank	Cat#00620
SW948 Cells	ATCC	Cat#CCL-237
SW1417 Cells	ATCC	Cat#CCL-238
SW1463 Cells	ATCC	Cat#CCL-234
Oligonucleotides
5’-TCTAGAGCGGCCGCAGGTACCAGTGAACGGACAGGGACTC-3’	Integrated DNA Technologies	N/A
5’-GGCCTTAGTTATTCAGGTACCTCAGCTACCTGTGATGCC-3’	Integrated DNA Technologies	N/A
Recombinant DNA
pG5luc	Promega	Cat#E2440
pBIND	Promega	Cat#E2440
pETDuet-1-PXR LBD-mSRC-1	Lin et al.32	N/A
pcDNA3-FLAG-PXR	Lin et al.45	N/A
pBIND-PXR LBD	This paper	N/A
pBIND-FXR LBD	This paper	N/A
pBIND-RORγ LBD	This paper	N/A
Software and algorithms
XDS	Kabsch48	https://xds.mr.mpg.de/
Phenix	Liebschner et al.52	https://phenix-online.org/
Coot	Emsley et al.51	http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMOL	Schrödinger	https://pymol.org/2/
Prism 9	Graphpad Software	https://www.graphpad.com/features