KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Biological samples | ||
| Bivalent HCW Sera | 20 | N/A |
| 3-dose HCW Sera | 1, 30 | N/A |
| Omicron BA.4/5-wave Infected First Responders and Household Contacts Sera | 1, 30 | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Transporter 5 Transfection Reagent | Polysciences | Cat# 26008-5 |
| Dulbecco’s Modified Eagles Medium (DMEM) | Sigma-Aldrich | Cat#: 11965-092 |
| Fetal Bovine Serum (FBS) | Thermo Fisher Scientific | Cat#: F1051 |
| 0.05% Trypsin +0.53 mM EDTA | Corning | Cat# 25-052-CI |
| Penicillin-Streptomycin | HyClone | Cat#: SV30010 |
| QIAprep Spin Miniprep Kit | QIAGEN | Cat# 27106 |
| Coelenterazine | GoldBio | Cat#: CZ2.5, CAS: 55779-48-1 |
| Deposited data | ||
| NT50 Values and De-identified patient data | SeroNet Coordinating Center, NCI, NIH | N/A |
| Experimental models: Cell lines | ||
| HEK293T | ATCC | Cat#: CRL-11268, RRID: CVCL_1926 |
| HEK293T-ACE2 | BEI Resources | Cat#: NR-52511, RRID: CVCL_A7UK |
| CaLu-3 | ATCC | RRID: CVCL_0609 |
| Recombinant DNA | ||
| pNL4-3-inGluc | David Derse, NIH47 | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_D614G | GenScript Biotech48 | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2 | GenScript Biotech30 | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5 | GenScript Biotech1 | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_XBB.1.5 | GenScript Biotech20 | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_XBB.1.5-E180V | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_XBB.1.5-K478R | This paper | N/A |
| pcDNA3.1-SARS-CoV-2-Flag-S-Flag_XBB.1.16 | This paper | N/A |
| Software and algorithms | ||
| GraphPad Prism | Version 9.0.0 | GraphPad |
| Other | ||
| Cytation 5 Imaging Reader | BioTek | N/A |