KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-APC7 C-term | Bethyl | Cat# A302-551A, RRID:AB_1998911 |
| Rabbit anti-APC1 | Bethyl | Cat# A301-653A-T, RRID:AB_2632162 |
| Mouse monoclonal (DH01) anti-FZR1/CDH1 | Thermo Fisher Scientific | Cat# MA5-11496, RRID:AB_10979615 |
| Rat monoclonal anti-Ki-67 (SolA15) | Thermo Fisher Scientific | Cat# 14-5698-82, RRID:AB_10854564 |
| Purified Mouse anti-Aim1 / Aurora B (IF, IB) | BD Biosciences | Cat# 611082, RRID:AB_2227708 |
| Rabbit monoclonal anti-Aurora B (IHC) | Abcam | Cat# ab287960, RRID: not found |
| Anti-H3S10ph (IF neurons) | Millipore | Cat# 06-570, RRID:AB_310177 |
| Anti-H3S10ph (IHC) | Cell Signaling Technology | Cat# 9701, RRID: AB_331535 |
| Rabbit polyclonal anti-H3S10ph (IF mitosis) | Active Motif | Cat# 39253, RRID:AB_2793206 |
| Rabbit monoclonal anti-Survivin | Cell Signaling Technology | Cat# 71G4B7, RRID:AB_2063948 |
| Rabbit monoclonal anti-Top2a | Abcam | Cat# ab52934, RRID:AB_883143 |
| Rabbit anti-H3K9me3 | Active Motif | Cat# 39286, RRID: AB_2935892 |
| Rabbit monoclonal anti-H3K27me3 | Cell Signaling Technology | Cat# 9733S, RRID:AB_2616029 |
| Mouse anti-H4K20me3 | Abcam | Cat# ab78517, RRID:AB_1951279 |
| Rabbit anti-Incenp (IB) | Proteintech | Cat# 29419-1-AP, RRID:AB_2918303 |
| Mouse anti-INCENP | Active Motif | Cat# 39259, RRID:AB_2715518 |
| Rabbit polyclonal anti-H3K27ac | Active Motif | Cat# 39034, RRID:AB_2722569 |
| Rabbit anti-HP1a | Cell Signaling Technology | Cat# 2616S, RRID:AB_2070987 |
| Rabbit anti-nucleolin | Bethyl | Cat# IHC-00083, RRID:AB_2267070 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary, Alexa Fluor 568 | Thermo Fisher Scientific | Cat# A-11031, RRID:AB_144696 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11006, RRID:AB_2534074 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary, Alexa Fluor 568 | Thermo Fisher Scientific | Cat# A-11007, RRID:AB_2896333 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21206, RRID:AB_2535792 |
| Donkey Anti-Mouse IgG IRDye 680 Conjugated antibody | LI-COR Biosciences | Cat# 926-32222, RRID:AB_621844 |
| IRDye 800CW Donkey anti-Rabbit IgG antibody | LI-COR Biosciences | Cat# 925-32213, RRID:AB_2715510 |
| IRDye 800CW Goat anti-Rat IgG antibody | LI-COR Biosciences | Cat# 926-32219, RRID:AB_1850025 |
| AffiniPure Rabbit Anti-Rat IgG (H+L) | Jackson Immuno Research | Cat# 312-005-045, RRID:AB_2339803 |
| OmniMap DAB anti-Rb | Ventana Medical systems | Cat# 05266548001, RRID: Not Found |
| Chemicals, Peptides, and Recombinant Proteins | ||
| UBE1 (E1) | (Jarvis et al., 201627) | N/A |
| CDH1 protein | (Jarvis et al., 201627) | N/A |
| UBCH10 (E2) | (Jarvis et al., 201627) | N/A |
| UBE2S (E2) | (Jarvis et al., 201627) | N/A |
| Fluorescent cyclin B1-NTD (CycB-NTD) | (Jarvis et al., 201627) | N/A |
| ubiquitin | (Jarvis et al., 201627) | N/A |
| Ki-67 AA 1-300 | (Ferguson et al., 20229) | N/A |
| Benzonase | Millipore | E1014 |
| Doxycycline Hyclate, 98% | Thermo Scientific | AC446060050 |
| Hoechst 33258 solution | Millipore Sigma | Cat# 94403 |
| TMT11 reagent | Thermo Fisher Scientific | A37727 |
| TMT16 reagent | Thermo Fisher Scientific | A44520 |
| TMTpro | Thermo Fisher Scientific | A44520 |
| Pierce Trypsin Protease, MS Grade | Thermo Fisher Scientific | Cat#90305 |
| Acetonitrile | Honeywell | AS017-0100 |
| Lys-C, Mass Spectrometry Grade | Wako Chemicals | 129-02541 |
| Palbociclib | Selleck Chemical LLC | 50-854-7 |
| Pierce Phosphatase Inhibitor Mini Tablets (TMT/MS) | Thermo Fisher Scientific | A32957 |
| Pierce Protease Inhibitor Mini Tablets (TMT/MS) | Thermo Fisher Scientific | A32953 |
| Protease inhibitor cocktail | Sigma | P8340 |
| Phosphatase inhibitor cocktail #3 | Sigma | P0044 |
| PMSF | Sigma | PMSF-RO |
| Polybrene | EMD Millipore | TR-1003-G |
| Puromycin | Thermo Fisher Scientific | J67236.XF |
| PEG-it Virus Precipitation Solution | System Biosciences | LV810A-1 |
| HyClone Fetal Bovine Serum | Cytiva | SH30071.03IH25-40 |
| Pierce BCA Protein Assay | Thermo Fisher Scientific | Cat# 23223 |
| Bradford regent | Bio-Rad | Cat# 5000006 |
| Revert total protein stain | LI-COR | Cat# 926-11011 |
| iScript cDNA synthesis kit | Bio-Rad | Cat# 1708890 |
| Lenti-X TM Packaging Single Shots (VSV-G) | Takara Bio | Cat# 631275 |
| Barasertib (AZD1152) | MedChemExpress | Cat# HY-10127 |
| Hesperadin | Cayman Chemical | Cat# 24199 |
| Deposited Data | ||
| Investigation of cerebellum-specific knockouts of Cdh1 and Apc7 | This Study (Table S2) | (http://www.ebi.ac.uk/pride) PXD038778 |
| Phosphoproteomic analysis of Aurora B inhibition in WT and CDH1 mutant RPE1 cells | This Study (Table S3) | (http://www.ebi.ac.uk/pride) PXD044042 |
| Investigating the effect of Aurora B inhibition in WT and Cdh1 mutant primary neuron cultures | This Study (Table S4) | (http://www.ebi.ac.uk/pride) PXD045116 |
| Original/source data | Mendeley Data | DOI: 10.17632/5f5mhb8yzv.1 |
| Experimental Models: cell lines | ||
| RPE-1 CEP192-mNeonGreen TetOn-Cas9 | (Meitinger et al., 202072) | ODCL0191 |
| RPE-1 CEP192-mNeonGreen TetOn-Cas9 ANAPC7 gRNA #4 | (Ferguson et al., 20229) | N/A |
| RPE-1 CEP192-mNeonGreen TetOn-Cas9 FZR1 gRNA #1 | This study | N/A |
| RPE-1 CEP192-mNeonGreen TetOn-Cas9 FZR1 gRNA #2 | This study | N/A |
| RPE-1 CEP192-mNeonGreen TetOn-Cas9 FZR1 gRNA #3 | This study | N/A |
| RPE-1 CEP192-mNeonGreen TetOn-Cas9 FZR1 gRNA #4 | This study | N/A |
| Experimental Models: Organisms/Strains | ||
| Anapc7 mutant on hybrid C57BL/6 x 129 | (Ferguson et al., 20229) | |
| Anapc7 mutant on Jackson C57BL/6 | (Ferguson et al., 20229) | N/A |
| Cdc20f/f mice on C57BL/6 | (Manchado et al., 201019) | N/A |
| Fzr1/f mice on C57BL/6 | (García-Higuera et al., 200818) | N/A |
| Math1/Atoh1-cre mice on C57BL/6 | The Jackson Laboratory | Cat# 011104 |
| Mki67 mutant on C57BL/6 | (Sobecki et al., 201636) | N/A |
| Wild-type Jackson C57BL/6 | The Jackson Laboratory | Cat# 000664 |
| Oligonucleotides | ||
| See Table S1 | ||
| Recombinant DNA/Cloning | ||
| pGEX-INCENP amino acids 256-632 | This study | N/A |
| pGEX-INCENP amino acids 256-563 | This study | N/A |
| pGEX-INCENP amino acids 256-563, Δ493-496, Δ539-542 | This study | N/A |
| NEB 5-alpha Competent E.Coli (High efficiency) | New England Biolabs | Cat# C2987H |
| Monarch Plasmid Miniprep kit | New England Biolabs | Cat# T1010S |
| BamHI- HF | New England Biolabs | Cat# R3136T |
| NotI-HF | New England Biolabs | Cat# R3189S |
| Esp3I | Thermo Scientific | Cat# FERER0451 |
| Monarch PCR and DNA Cleanup Kit | New England Biolabs | Cat# T1030S |
| Monarch DNA Gel Extraction Kit | New England Biolabs | Cat# T1020S |
| Software and Algorithms | ||
| AlphaFold | (Jumper et al., 202129) | https://alphafold.ebi.ac.uk |
| ImageJ | (Schneider et al., 201284) | https://imagej.nih.gov/ij/ |
| NIS - Elements | Nikon | RRID:SCR_014329 |
| Image Studio | LI-COR | N/A |
| ImageLab | Bio-rad | N/A |
| CFX Maestro | Bio-Rad | N/A |
| SEQUEST-based in-house platform | Thermo Fisher Scientific (Eng et al., 199478; Huttlin et al., 201079) | http://fields.scripps.edu/yates/wp/?page_id=17 |
| Other | ||
| Ventana Discovery Ultra | Roche Diagnostics | RRID: SCR_021254 |
| Rneasy Mini Kit | Qiagen | Cat# 74104 |
| Sep-Pak cartridge | Waters | WAT054925 |
| Fe-NTA spin column | Thermo Fisher | A32992 |