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. Author manuscript; available in PMC: 2024 Dec 7.
Published in final edited form as: Mol Cell. 2023 Nov 9;83(23):4304–4317.e8. doi: 10.1016/j.molcel.2023.10.028

Figure 5. SAC inhibits YTHDF1 by suppressing YTHDF1 condensation.

Figure 5

(A) Analysis of YTHDF1 (left) or YTHDF2 (right) protein oligomers in cultured mouse neurons treated with SAC. YTHDF protein oligomers were visualized by Native-PAGE and loading controls were performed with denaturing SDS-PAGE.

(B) SAC treatment rescues aberrant YTHDF1 condensation and protein synthesis in Fmr1 KO neurons. Protein synthesis rates were measured by HPG incorporation in mouse neurons (n = 6 for each condition).

(C) Box plots showing the log2-transformed fold changes of the translation efficiency of YTHDF1 target transcripts (DF1 target) or non-targets (NT) in WT or Fmr1 KO mouse neurons upon SAC.

(B) and (C), P values were determined using a Wilcoxon ranked sum test. NS means not significant (P values > 0.05). Reported P values are for the individual comparisons. Box-plot elements: centre line, median; box limits, upper and lower quartiles; whiskers, 1–99%; error bars, 95% confidence interval of mean