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. Author manuscript; available in PMC: 2025 Jan 1.
Published in final edited form as: J Control Release. 2023 Dec 7;365:602–616. doi: 10.1016/j.jconrel.2023.11.034

Fig. 8. miR-511-3p illustrates a direct binding to C3 and regulates C3 activity.

Fig. 8.

(A) Schematic representation of miR-511-3p-C3 mRNA biotin pulldown assay. (B) qPCR analysis of C3 expression in miR-511-3p-C3 mRNA complexes. n=4. (C) In silico prediction of miR-511-3p binding site in C3 mRNA by BiBiServ RNAhybrid. (D-F) Dual-luciferase assay in HEK293T cells transfected with C3-psicheck2 plasmid containing the binding site (D), mutant binding site (E), and control (F) and then treated with either NC-mimics or miR-511-3p mimics. n=3. All data were expressed as the means ± SEM. Statistical significance was calculated by one-way ANOVA followed by Tukey’s post-hoc test. *P < 0.05, ***P < 0.001.