TABLE 1.

Effect of pyocyanin on cytokine-dependent increases in IL-8 release^a

Cell type	IL-8 (ng/mg protein)
	Pyo	TNF	TNF-Pyo	IL-1	IL-1–pyo
A549	55 ± 16	3,100 ± 138	6,400 ± 870	9,200 ± 310	11,700 ± 1,400
Calu-3	572 ± 106	790 ± 130	1,900 ± 340	3,600 ± 930	5,900 ± 870
ECFTE	23 ± 4	280 ± 71	860 ± 160	670 ± 270	1,470 ± 210
C38	8.1 ± 1.3	190 ± 40	330 ± 39	250 ± 28	1,270 ± 80
IB3	13 ± 0.5	1,930 ± 541	2,600 ± 300b	1,500 ± 170	2,890 ± 96

^aCells were exposed for 24 h to pyocyanin (Pyo) alone (5 μM) or to the indicated cytokine ± 5 μM pyocyanin. At the end of the incubation period, the medium was recovered and IL-8 was measured by ELISA. Values represent the average ± standard deviation of triplicate samples. Unless otherwise indicated, values for cytokines with pyocyanin are statistically different (P <0.05) from values for cytokines alone. Similar results were obtained for each cell type in two or more independent experiments. 

^bP < 0.1