Figure 2: Src inhibition protects Ca²⁺ signaling capacity against insult from VEGF₁₆₅, bFGF, PlGF, TNFα, and IL-1β.

Pretreatment with the Src inhibitor PP2 at 10uM for 20 minutes before treatment with a panel of growth factors and cytokines at 10ng/mL for 30 minutes offered protection of ATP-stimulated Ca²⁺ bursting responses in HUVEC. Stimulation with 100uM ATP only is depicted as Control in black bars, stimulation with ATP after growth factor or cytokine treatment is depicted in light gray bars, and ATP after PP2 pretreatment prior to growth factor or cytokine treatment is in dark gray bars. Data is shown as mean count of Ca²⁺ bursts in cells that responded with at least 3 Ca²⁺ bursts upon initial ATP stimulation, ± SEM for at least 80 cells from at least 4 separate dishes of pooled cells. Statistical analysis was done by Rank-sum test, with * indicating p<0.05 and Kruskal-Wallis with + indicating p<0.05.