HUVEC were pretreated with the MEK/ERK inhibitor U0126 or Src inhibitor PP2 at 10um 30 minutes before treatment with a panel of cytokines (TNFα, IL-1β, IL-6 and IL-8) at 10ng/mL. Data is shown as mean ± SEM, with resistance normalized to control in the top row and the quantitative change in resistance relative to control at hours 5, 10 ,15 and 20 in the bottom row. Data was collected for 24 hours after growth factor and cytokine treatments. Treatments were run in triplicate with n=6 96 well plates. Statistical analysis was done by student’s t test with * indicating p<0.05 reduction in resistance measurements.