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. Author manuscript; available in PMC: 2024 Feb 16.
Published in final edited form as: Biochemistry. 2023 Dec 18;63(1):141–151. doi: 10.1021/acs.biochem.3c00564

Figure 2.

Figure 2.

NanoDSF Tm determination experiments for (A) PglJ Cc 33237 (blue), (B) PglJ Cc 13826 (purple), and (C) PglJ Cj 11168 (green) tested against 1 mM UDP and a panel of UDP-sugars. Each data point represents the mean value derived from independent measurements (n = 2–3) with error bars indicating standard deviation (± SD). The unliganded PglJ Cc 33237 exhibited a melting point, Tm of 41.0 ± 0.2 °C, while PglJ Cc 13826 demonstrated a Tm of 47.5 ± 0.1 °C. Note that the melting transition for Cj PglJ, with UDP-GalNAc occurred at two points: 45.2 ± 0.1°C and at 51.4 ± 0.2°C.