Figure 2. H3K9me2 depletion in young mice impairs alveolar progenitor activity.

(a) Schematic of in vitro G9ai organoid experiment. (b) Representative fluorescent Images of day 14 alveolar organoid cultures +/- G9ai. Scale bar =100 μm. (c) Quantification of organoid forming efficiencies from Sca-1⁻ progenitors +/- G9ai. *=p<0.05, T-test, n=3. (d) Quantification of Pdpn+ organoid % relative to all alveolar organoids, n=4. (e) Schematic of stromal cell in vitro G9ai experiment. (f) Quantification of organoid forming efficiencies from Sca-1⁻ progenitors co-cultured stromal cells pre-treated +/- G9ai, n=f. (g) Schematic of in vitro Adeno-Cre organoid experiment. (h) Representative images of SPC⁺ organoids derived from Adeno-Cre infected Sca-1⁻ progenitors from G9a^wt/wt and G9a^fl/fl mice, stained for SPC. Scale bar = 100 μm. (i) Quantification of organoid forming efficiencies from Adeno-Cre infected Sca-1⁻ progenitors from G9a^wt/wt and G9a^fl/fl mice. *=p<0.05, T-test, n=3. (j) Quantification of mean size of organoid derived from Adeno-Cre infected Sca-1⁻ progenitors from G9a^wt/wt and G9a^fl/fl mice. *=p<0.05, T-test, n=3. (k) Representative images of organoids from Adeno-Cre infected Sca-1⁻ progenitors from G9a^wt/wt and G9a^fl/fl mice, immunostained for the indicated proteins. (l) Quantification of Pdpn+ organoid % relative to all alveolar organoids generated from G9a^wt/wt and G9a^fl/fl Sca-1⁻ progenitors, n=3.