KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
| Antibodies | ||
| Goat polyclonal anti-PDGFRα | R&D Systems | Cat# AF1062; RRID: AB_2236897 |
| Mouse monoclonal anti-CC1 | Millipore | Cat# OP80; RRID: AB_2057371 |
| Rabbit polyclonal anti-BMAL1 | Novus Biologicals | Cat# NB100–2288; RRID: AB_10000794 |
| Chicken polyclonal anti-Tyrosine Hydroxylase (TH) | Aves lab | Cat# TYH; RRID: AB_10013440 |
| Goat polyclonal anti-CD31/PECAM1 | R&D Systems | Cat# AF3628; RRID: AB_2161028 |
| Rat monoclonal anti-CD13 | Biorad | Cat# MCA2183; RRID: AB_323691 |
| Rat monoclonal anti-myelin basic protein (MBP) | Abcam | Cat# ab7349; RRID: AB_305869 |
| Rat monoclonal anti-CD140A | BD Pharmingen | Cat# 558774; RRID: AB_397117 |
| Rabbit polyclonal anti-NG2 Chondroitin Sulfate Proteoglycan | Millipore | Cat# AB5320; RRID: AB_11213678 |
| Rat monoclonal anti-GAPDH | BioLegend | Cat# 607903; RRID: AB_2734504 |
| Alexa 488 sheep anti-mouse IgG | Jackson Immunoresearch | Cat# 515-545-072; RRID: AB_2340335 |
| Alexa 594 donkey anti-mouse IgG | Jackson Immunoresearch | Cat# 715-585-150; RRID: AB_2340854 |
| Alexa 647 donkey anti-mouse IgG | Jackson Immunoresearch | Cat# 715-605-150; RRID: AB_2340862 |
| Alexa 488 donkey anti-rabbit IgG | Jackson Immunoresearch | Cat# 711-546-152; RRID: AB_2340619 |
| Alexa 594 donkey anti-rabbit IgG | Jackson Immunoresearch | Cat# 711-586-152; RRID: AB_2340622 |
| Alexa 647 donkey anti-rabbit IgG | Jackson Immunoresearch | Cat# 711-605-152; RRID: AB_2492288 |
| Alexa 488 donkey anti-goat IgG | Jackson Immunoresearch | Cat# 705-545-147; RRID: AB_2336933 |
| Alexa 594 donkey anti-goat IgG | Jackson Immunoresearch | Cat# 705-586-147; RRID: AB_2340434 |
| Alexa 647 donkey anti-goat IgG | Jackson Immunoresearch | Cat# 705-606-147; RRID: AB_2340438 |
| Alexa 488 donkey anti-rat IgG | Jackson Immunoresearch | Cat# 712-545-153; RRID: AB_2340684 |
| Alexa 594 goat anti-rat IgG | Jackson Immunoresearch | Cat# 112-005-167; RRID: AB_2338101 |
| Alexa 647 donkey anti-rat IgG | Jackson Immunoresearch | Cat# 712-586-150; RRID: AB_2340690 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Lysolecithin (L-α-Lysophosphatidylcholine) | Sigma-Aldrich | L4129; CAS: 9008-30-4 |
| Avertin (tribromoethanol) | Sigma-Aldrich | T48402, CAS: 75-80-9 |
| 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI) | Thermo Fisher | 62247, CAS: N.A. |
| ProLong Gold mounting medium | Life Technologies | P36930, CAS: N.A. |
| Glutaraldehyde | EMS | 16000, CAS: 111-30-8 |
| Paraformaldehyde | EMS | 15710, CAS: 30525-89-4 |
| Sodium cacodylate | EMS | 12300, CAS: 6131-99-3 |
| Epoxy resin EMbed-812 | EMS | 14120, CAS: N.A. |
| Fluorescein sodium salt | Sigma-Aldrich | 46960, CAS: 518-47-8 |
| Poly-D-lysine | Sigma-Aldrich | P6407, CAS: 27964-99-4 |
| Papain | Worthington | Cat# LS003126, CAS: N.A. |
| Trypsin | Sigma-Aldrich | Cat# T9935, CAS: N.A. |
| Dulbecco’s Modified Eagle’s Medium (high glucose) | Thermo Fisher | 11960–044, CAS: N.A. |
| Neurobasal medium | Gibco | Cat# 21103–049, CAS: N.A. |
| Earle’s balanced salt solution | Sigma-Aldrich | Cat# E7510, CAS: N.A. |
| Fetal calf serum | GIBCO | Cat# 10437–028, CAS: N.A. |
| Dulbecco’s PBS (dPBS) | GIBCO | Cat# 14040182, CAS: N.A. |
| Penicillin/ Streptomycin | Gibco | 15140–122, CAS: N.A. |
| Glutamine | Thermo Fisher | 25030–081, CAS: 56-85-9 |
| Sodium pyruvate | Thermo Fisher | 11360–070, CAS: 113-24-6 |
| Insulin | Sigma-Aldrich | I6634, CAS: 11061-68-0 |
| N-Acetyl-L-cysteine | Sigma-Aldrich | A8199, CAS: 616-91-1 |
| Trace Elements B | Corning | 25022CI, CAS: N.A. |
| d-Biotin | Sigma-Aldrich | B4639, CAS: 58-85-5 |
| Bovine Serum Albumin | Sigma-Aldrich | A4161, CAS: 9048-46-8 |
| Transferrin | Sigma-Aldrich | T1147, CAS: 11096-37-0 |
| Putrescine | Sigma-Aldrich | P5780, CAS: 333-93-7 |
| Progesterone | Sigma-Aldrich | P8783, CAS: 57-83-0 |
| Sodium selenite | Sigma-Aldrich | S5261, CAS: 10102-18-8 |
| Forskolin | Sigma-Aldrich | F6886, CAS: 66575-29-9 |
| CNTF | Peprotech | 450–13, CAS: N.A. |
| PDGF-AA | Peprotech | 100–13A, CAS: N.A. |
| NT-3 | Peprotech | 450–03, CAS: 130939-66-1 |
| B-27 | Thermo Fisher | 17504–044, CAS: 128446-35-5 |
| T3 | Sigma-Aldrich | T6397, CAS: 6893-02-3 |
| Phalloidin | Thermo Fisher | A12379, CAS: 17466-45-4 |
| Bolt 4–12% Bis-Tris Plus Gel | Thermo Fisher | NW04120, CAS: N.A. |
| Nitrocellulose membrane | Thermo Fisher | IB23002, CAS: N.A. |
| Dexamethasone | Sigma-Aldrich | D4902, CAS: 50-02-2 |
| Aphidicolin | Sigma-Aldrich | 178273, CAS: 38966-21-1 |
| Beetle luciferin potassium salt | Promega | E1602, CAS: 115144-35-9 |
| QIAzol Lysis Reagent | Qiagen | 79306, CAS: N.A. |
| BSL1 | Vector Laboratories | L1100, CAS: N.A. |
| Critical commercial assays | ||
| SuperScript™ III First-Strand Synthesis System | Thermo Fisher | 18080051, CAS: N.A. |
| Power Up SYBR Green Master Mix | Applied Biosystems | A25776, CAS: N.A. |
| NEBNext Ultra II RNA Library Prep | NEB | E7775, CAS: N.A. |
| Click-iT EdU cell proliferation kit | Life Technologies | C10339, CAS: N.A. |
| Deposited data | ||
| mRNA-seq, OPCs, Bmal1 WT, ZT0, rep 1 | This paper | GSM7667057 |
| mRNA-seq, OPCs, Bmal1 WT, ZT0, rep 2 | This paper | GSM7667058 |
| mRNA-seq, OPCs, Bmal1 WT, ZT0, rep 3 | This paper | GSM7667059 |
| mRNA-seq, OPCs, Bmal1 WT, ZT0, rep 4 | This paper | GSM7667060 |
| mRNA-seq, OPCs, Bmal1 WT, ZT6, rep 1 | This paper | GSM7667061 |
| mRNA-seq, OPCs, Bmal1 WT, ZT6, rep 2 | This paper | GSM7667062 |
| mRNA-seq, OPCs, Bmal1 WT, ZT6, rep 3 | This paper | GSM7667063 |
| mRNA-seq, OPCs, Bmal1 WT, ZT6, rep 4 | This paper | GSM7667064 |
| mRNA-seq, OPCs, Bmal1 WT, ZT6, rep 5 | This paper | GSM7667065 |
| mRNA-seq, OPCs, Bmal1 WT, ZT12, rep 1 | This paper | GSM7667066 |
| mRNA-seq, OPCs, Bmal1 WT, ZT12, rep 2 | This paper | GSM7667067 |
| mRNA-seq, OPCs, Bmal1 WT, ZT12, rep 3 | This paper | GSM7667068 |
| mRNA-seq, OPCs, Bmal1 WT, ZT12, rep 4 | This paper | GSM7667069 |
| mRNA-seq, OPCs, Bmal1 WT, ZT12, rep 5 | This paper | GSM7667070 |
| mRNA-seq, OPCs, Bmal1 WT, ZT12, rep 6 | This paper | GSM7667071 |
| mRNA-seq, OPCs, Bmal1 WT, ZT18, rep 1 | This paper | GSM7667072 |
| mRNA-seq, OPCs, Bmal1 WT, ZT18, rep 2 | This paper | GSM7667073 |
| mRNA-seq, OPCs, Bmal1 WT, ZT18, rep 3 | This paper | GSM7667074 |
| mRNA-seq, OPCs, Bmal1 WT, ZT18, rep 4 | This paper | GSM7667075 |
| mRNA-seq, OPCs, Bmal1 KO, ZT0, rep 1 | This paper | GSM7667076 |
| mRNA-seq, OPCs, Bmal1 KO, ZT0, rep 2 | This paper | GSM7667077 |
| mRNA-seq, OPCs, Bmal1 KO, ZT0, rep 3 | This paper | GSM7667078 |
| mRNA-seq, OPCs, Bmal1 KO, ZT0, rep 4 | This paper | GSM7667079 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 1 | This paper | GSM7667080 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 2 | This paper | GSM7667081 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 3 | This paper | GSM7667082 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 4 | This paper | GSM7667083 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 5 | This paper | GSM7667084 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 6 | This paper | GSM7667085 |
| mRNA-seq, OPCs, Bmal1 KO, ZT6, rep 7 | This paper | GSM7667086 |
| mRNA-seq, OPCs, Bmal1 KO, ZT12, rep 1 | This paper | GSM7667087 |
| mRNA-seq, OPCs, Bmal1 KO, ZT12, rep 2 | This paper | GSM7667088 |
| mRNA-seq, OPCs, Bmal1 KO, ZT12, rep 3 | This paper | GSM7667089 |
| mRNA-seq, OPCs, Bmal1 KO, ZT12, rep 4 | This paper | GSM7667090 |
| mRNA-seq, OPCs, Bmal1 KO, ZT18, rep 1 | This paper | GSM7667091 |
| mRNA-seq, OPCs, Bmal1 KO, ZT18, rep 2 | This paper | GSM7667092 |
| mRNA-seq, OPCs, Bmal1 KO, ZT18, rep 3 | This paper | GSM7667093 |
| mRNA-seq, OPCs, Bmal1 KO, ZT18, rep 4 | This paper | GSM7667094 |
| Experimental models: Cell lines | ||
| Experimental models: Organisms/strains | ||
| Mouse: B6;FVB-Ifi208Tg(Cspg4-cre)1Akik/J | The Jackson Laboratory | RRID: IMSR_JAX: 008533 |
| Mouse: B6N.Cg-Tg(Pdgfra-cre/ERT)467Dbe/J | The Jackson Laboratory | RRID: IMSR_JAX: 018280 |
| Mouse: B6.129S4(Cg)-Bmal1tm1Weit/J | The Jackson Laboratory | RRID: IMSR_JAX: 007668 |
| Mouse: B6.129S6-Per2tm1Jt/J | The Jackson Laboratory | RRID: IMSR_JAX: 006852 |
| Oligonucleotides | ||
| Oligonucleotides for genotyping | See Table S5 | N.A. |
| Oligonucleotides for RT-qPCR analysis | See Table S5 | N.A. |
| Recombinant DNA | ||
| Software and algorithms | ||
| ImageJ | NIH | https://imagej.net/Downloads |
| Incucyte ZOOM | Essen Bioscience | https://www.essenbioscience.com/es/resources/incucyte-zoom-resources-support/software-modules-incucyte-zoom/ |
| VitalView | Mini Mitter | https://www.starrlifesciences.com/resource/vitalview-software-download/ |
| SleepSign | Kissei Comtec Co. Ltd. | https://sleepsign-for-animal.software.informer.com/download/ |
| CatWalk XT 9.0 | Noldus Information Technology | https://catwalk-xt.software.informer.com/ |
| Ethovision XT | Noldus Information Technology | https://ethovision-xt.software.informer.com/ |
| ZenBlue | Zeiss | https://www.zeiss.com/microscopy/en_us/products/microscope-software/zen.html |
| Image Studio Lite | LI-COR | https://www.licor.com/bio/image-studio-lite/ |
| Imaris for Neuroscientists Cell Imaging Software ver. 9.8. | Oxford Instruments | https://imaris.oxinst.com/products/imaris-for-neuroscientists |
| GraphPad Prism | Dotmatics | https://www.graphpad.com:443/ |
| Hisat2 v2.0.5 | N.A. | http://daehwankimlab.github.io/hisat2/ |
| FeatureCounts v1.5.0-p3 | N.A. | https://subread.sourceforge.net/featureCounts.html |
| Profiler R package | N.A. | https://cran.r-project.org/web/packages/profileR/index.html |
| TwoSampleMR package version 0.5.6 with R version 4.1.3. | N.A. | https://mrcieu.github.io/TwoSampleMR/news/index.html |
| mRnd | N.A. | https://shiny.cnsgenomics.com/mRnd/ |
| Other | ||
| Telemetry device G2 E-Mitter | Mini Mitter | G2 E-Mitter |
| Low-torque slip-ring commutator | Biella Engineering | N.A. |
| Glass amplifier | West Warwick | N.A. |
| Sleep recording system Vital Recorder | Kissei Comtec Co. Ltd. | N.A. |
| Confocal microscope Zeiss LSM900 | Zeiss | LSM900 |
| Transmission Electron Microscope | Jeol USA | JEOL JEM-1400 TEM |
| Microplate reader | Molecular Devices | SpectraMax iD3 |
| Peristaltic pump | VWR | 70730–062 |
| WesternBright Sirius | Advansta | K-12043-C20 |
| QuantStudio™ 6 Flex Real-Time PCR System | Applied Biosystems | N.A. |
| Boyden chambers | Corning | CLS3422 |
| NovaSeq 6000 S4 system | Illumina | N.A. |