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. Author manuscript; available in PMC: 2024 Feb 16.
Published in final edited form as: Cell Rep. 2023 Oct 19;42(10):113274. doi: 10.1016/j.celrep.2023.113274

Figure 7. Cntnap1C324R and Cntnap1R765C mutant proteins remain in the neuronal soma and show reduced binding with contactin.

Figure 7.

(A–D) Immunostaining of spinal cord cross-sections. Scale bar: 5 μm.

(E) Schematic showing protein domains in Cntnap1 and Contactin.

(F) Co-immunoprecipitation from spinal cord lysates.

(G–I) Representative immunostaining images of Cntnap1 and Contactin co-staining in live non-permeable co-transfected HEK cells. Scale bar: 5 μm.

(J) Co-immunoprecipitation of Cntnap1 and Contactin from HEK cells.

(K) Cell surface/membrane expression of Cntnap1 and Contactin in HEK cells.

(L–N) Cell surface/membrane expression of Cntnap1 (WT) or mutant C324R or R765C in HEK cells co-transfected with cDNAs for Cntnap1 (WT) or Cntnap1 mutants and Contactin. EGFR was used as the cell surface protein marker, and GAPDH was used as negative control for cell surface expression. Data are represented as the mean ± SEM of three independent experiments.