Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| LTL lectin-FITC conjugate | Vector Laboratories | Cat# FL-1321 |
| Goat polyclonal anti-CA4(Car4) | R&D | Cat# AF2414 |
| Rabbit polyclonal anti-SGLT2 (Slc5a2) | Abcam | Cat# ab85626 |
| Rabbit monoclonal anti- Aquaporin 1 | Abcam | Cat# ab168387 |
| Chemicals, peptides, and recombinant proteins | ||
| Testosterone | Sigma-Aldrich | T1500; CAS: 58-22-0 |
| Probes for RNA in situ | ||
| RNAscope® Probe- Mm-Prlr-C2 | Advanced Cell Diagnostics | 430791-C2 |
| RNAscope® Probe- Mm-Gsta4-C1 | Advanced Cell Diagnostics | 1132411-C1 |
| RNAscope® Probe- Mm-Slco1a1 | Advanced Cell Diagnostics | 831051 |
| RNAscope® Probe- Mm-Hao2-C1 | Advanced Cell Diagnostics | 1201591-C1 |
| RNAscope® Probe- Mm-Slco1a1 | Advanced Cell Diagnostics | 831051 |
| RNAscope® Probe- Mm-Atp11a-C3 | Advanced Cell Diagnostics | 489841-C3 |
| RNAscope® Probe- Mm-Abcc3-C3 | Advanced Cell Diagnostics | 1201571-C3 |
| RNAscope® Probe- Mm-Cyp2j13-C3 | Advanced Cell Diagnostics | 1201581-C3 |
| RNAscope® Probe- Mm-Cyp7b1-C2 | Advanced Cell Diagnostics | 471001-C2 |
| Deposited data | ||
| Raw and analyzed bulk RNA-seq data | This paper | GEO: GSE225622 |
| Raw and processed multimodal data | This paper | GEO: GSE225566 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | JAX: 000664 |
| Mouse: B6(Cg)-Esr1tm4.1Ksk/J | The Jackson Laboratory | JAX: 032173 |
| Mouse: B6(Cg)-Esr1tm4.1Ksk/J | The Jackson Laboratory | JAX: 032173 |
| Mouse: B6.129S1-Artm2.1Reb/J | The Jackson Laboratory | JAX: 018450 |
| Mouse: C57BL/6 Mice | The Charles River Laboratories | C57BL/6NCrl inbred |
| Software and algorithms | ||
| DPGP | McDowell et al.57 | https://github.com/PrincetonUniversity/DP_GP_cluster |
| DoRothEA | Garcia-Alonso et al.58 Holland et al.8/9/2023 3:07:00 PM |
https://bioconductor.org/packages/release/data/experiment/html/dorothea.html |
| ChEA3 | Keenan et al.60 | https://maayanlab.cloud/chea3/ |
| QuPath | Bankhead et al.111 | https://github.com/qupath/qupath |
| fastq | Chen et al.112 | https://github.com/OpenGene/fastp |
| STAR | Dobin et al.113 | https://code.google.com/archive/p/rna-star/ |
| FeatureCounts | Liao et al.114 | https://subread.sourceforge.net |
| DESeq2 | Love et al.115 | http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html |
| Kallisto-Sleuth | Pimentel et al.116 | https://github.com/pachterlab/kallisto |
| PSI-Sigma | Lin et al.117 | https://github.com/wososa/PSI-Sigma |
| DRIMSeq | Love et al.118 | https://github.com/gosianow/DRIMSeq |
| Seurat | Hao et al.119 | https://satijalab.org/seurat/ |
| Signac | Stuart et al.120 | https://stuartlab.org/signac/ |
| SoupX | Young and Behjati121 | https://github.com/constantAmateur/SoupX |
| DoubletFinder | McGinnis et al.122 | https://github.com/chris-mcginnis-ucsf/DoubletFinder |
| ArchR | Granja et al.123 | https://github.com/GreenleafLab/ArchR |
| MACS2 | Zhang et al.124 | https://pypi.org/project/MACS2/ |
| sSeq | Yu et al.125 | https://bioconductor.org/packages/release/bioc/html/sSeq.html |
| MEME Suite | Bailey et al.126 | https://meme-suite.org/meme/ |
| bowtie2 | Langmead and Salzberg127 | https://github.com/BenLangmead/bowtie2 |
| SAMtools | Danecek et al.128 | https://github.com/samtools/samtools |
| bedGraphTobigWig | Kent et al.129 | https://www.encodeproject.org/software/bedgraphtobigwig/ |
| Customized Code | This paper | https://github.com/LingyunXiong/Kidney_SexDiff |