TABLE 2.
Effects of bacterial extract (supernatant of sonicate) and VacA cytotoxin-containing broth culture supernatant of a cagA(+) H. pylori strain on HT29-19A epithelial function in an Ussing chamber
| Prepn (no. of filters examined) | Mean ± SE
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Isc (μA/cm2) | PD (mV) | R (Ω/cm2) | JNa (μEq/h · cm2) | JMan (μmol/h · cm2) | J3H-HRP (ng/h · cm2) | JD (ng/h · cm2) | JHRPi (ng/h · cm2) | |
| Control (12) | 3.8 ± 0.7 | 0.4 ± 0.1 | 114 ± 12 | 5.1 ± 0.3 | 0.04 ± 0.00 | 1,947 ± 131 | 1,878 ± 137 | 78 ± 22 |
| Bacterial extract (12) | 4.9 ± 1.1 | 0.4 ± 0.0 | 104 ± 6 | 5.5 ± 0.3 | 0.04 ± 0.00 | 1,993 ± 162 | 1,928 ± 162 | 65 ± 4 |
| Control (21) | 2.0 ± 0.4 | 0.3 ± 0.1 | 143 ± 18 | 3.5 ± 0.3 | 0.04 ± 0.00 | 2,117 ± 139 | 2,050 ± 130 | 68 ± 12 |
| VacA supernatant (21) | 6.2 ± 0.4a | 1.1 ± 0.1a | 184 ± 13 | 2.6 ± 0.2 | 0.04 ± 0.00 | 2,031 ± 181 | 1,963 ± 181 | 68 ± 11 |
a
Significantly different from control monolayer value (P < 0.0001).