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. 2005 May;73(5):3188–3191. doi: 10.1128/IAI.73.5.3188-3191.2005

FIG. 3.

FIG. 3.

PIA production in ica-negative S. epidermidis wild-type and corresponding ica locus-expressed strains. PIA was extracted from 24-h cultures by boiling with 0.5% EDTA for 5 min. Three-μl samples of the extract were spotted on nitrocellulose membrane, and PIA production was assayed by immunodot blot and evaluated by densitometry as described (15). The experiment was performed twice. (A) Representative immunodot blot. (B) Bars show the mean ± standard error: 1, Tü3298-ica; 2, Tü3298; 3, ATCC 12228-ica; 4, ATCC 12228; 5, HB-ica; 6, HB.