Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 4;31(2):283–292. doi: 10.1038/s41594-023-01148-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, IDP ensembles with and without structural biases. In all schemes, a single conformation is shown in color and other conformations are shown in gray. Structural biases increase the density in specific regions of the ensemble and alter its average dimensions. b, FRET construct consisting of an IDP between two FPs that serve as a FRET donor and a FRET acceptor. c, In vitro experiments. Top: FRET. Middle: SAXS. Bottom: analytical SEC. d, In vitro solution space scanning measures the sensitivity of ensemble structure to changes in solution conditions. e, Live cell FRET microscopy is performed on cells expressing the same constructs used in vitro. f, Changes in ensemble dimensions are measured using live cell FRET following rapid hyperosmotic and hypoosmotic challenges. Hyper, hyperosmotic; Iso, isoosmotic; Hypo, hypoosmotic.