Fig. 5. Determination of FRET pair influence on IDP ensemble dimensions.

a, Original FRET construct (left) consisting of an IDP between two FPs that serve as a FRET donor and a FRET acceptor and the flipped construct (right) with the FRET pairs on the opposite ends. b, $E_{\mathrm{f}}^{\mathrm{app}}$ of selected constructs measured in vitro for the original (darker color) and flipped (lighter color) constructs. N = 24 for original and N = 12 for flipped constructs. c, $E_{\mathrm{f}}^{\mathrm{cell}}$ of selected constructs measured in HEK293T cells for original (darker color) and flipped (lighter color) constructs. Features are as in Fig. 2g. d, $\mathrm{\Delta }{E}_{\mathrm{f}}^{\mathrm{cell}}$ of original (darker color) and flipped (lighter color) constructs. Features are as in Fig. 2g,h. The dataset used to generate the live cell figures is in Supplementary Data 3. N for each violin plot is in Supplementary Data 4.

Source data