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Effect of the caspase-3 inhibitor Z-DEVD-FMK on Salmonella-induced DNA fragmentation. THP-1 cells were preincubated for 1 hour with 12 μM Z-DEVD-FMK in 0.6% dimethyl sulfoxide (DMSO), or the DMSO vehicle alone, and then infected with wild-type Salmonella serovar Dublin. After 5 h, the cells were harvested and processed for caspase-3 activity in cell lysates (A) and by FACS analysis for DNA fragmentation (B).
