FIG. 6.
Similar amounts of LouTat 1 VSG and 117 VSG are present in LT1:117 VSG double-expresser trypanosomes. Trypanosomes were metabolically labeled with [35S]methionine and [35S]cysteine in Met/Cys-depleted medium for 4 h. Cells were lysed, and the lysates were incubated with rabbit anti-LouTat 1 VSG or anti-117 VSG antibodies coupled to Sepharose A beads. Equivalent amounts of immunoprecipitated material were separated by SDS-PAGE and the bands detected by autoradiography. Band density was measured using GeneTools imaging software (Hitachi Genetic Systems; Hitachi Software Engineering Co., Ltd.). Scan pixel densities for precipitated LouTat 1 and 117 VSGs in autoradiograms were 1,422,050 and 1,036,078, respectively.