FIG. 3.

CD40 induces anti-T. gondii activity in mouse macrophages independently of IFN-γ but requires TNF-α signaling. (A) Peritoneal macrophages from BALB/c mice were cultured with either isotype control or stimulatory anti-CD40 MAb in the presence of a neutralizing anti-IFN-γ or control MAbs. Monolayers were examined by light microscopy 18 h after addition of T. gondii. (B) Peritoneal macrophages from BALB/c and IFN-γ^−/− mice were cultured with either isotype control or stimulatory anti-CD40 MAbs. (C) Peritoneal macrophages from BALB/c mice were cultured with either isotype control or stimulatory anti-CD40 MAb in the presence of a neutralizing anti-TNF-α or control MAbs. (D) Peritoneal macrophages from B6/129 and TNFR1/2^−/− mice were cultured with either isotype control or stimulatory anti-CD40 MAb. Results of one representative experiment out of three are shown.