FIG. 7.

CD40 does not induce NOS2 expression in mouse macrophages. (A) Bone marrow-derived macrophages were cultured in CM alone or in the presence of isotype control or stimulatory anti-CD40 MAb or IFN-γ plus LPS. Total cell lysates were obtained after 24 h and were used to determine levels of NOS2 and actin by immunoblot staining. (B) RNA was obtained from bone marrow-derived macrophages after 6 h and used to generate cDNA. Levels of NOS2 and HPRT mRNA were determined by reverse transcription-PCR. Results of one representative experiment out of three are shown.