FIG. 3.

Southern blot analysis showing conservation of sopD in different Salmonella serotypes. Total cell DNA was isolated from S. choleraisuis A57 (lane 1), S. dublin 2229 (lane 2), S. enteritidis 149 (lane 3), S. gallinarum 287/91 (lane 4), S. pullorum 449/87 (lane 5), and S. typhimurium 12/75 (lane 6) and digested with HpaI. A DNA fragment including the full-length sopD was amplified by PCR, labelled, and used as a probe. The positions of size markers (in kilobases) are indicated on the left. The sopD gene of S. dublin is known to contain one HpaI site.