TABLE 1.
Bacterial strains and plamsids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | recA | BRL |
| S17.1 | λpir RP4-2 Tc::Mu-Km::Tn7 | 21 |
| S. dublin | ||
| 2229 | Wild-type, virulent field isolate of S. dublin | IAHa |
| B1 | sipB::pDM4, Cmlr | 29 |
| B2 | ΔsipB | This study |
| SB2 | ΔsopB | 4 |
| B2SD1 | ΔsipB sopD::pDM4, Cmlr | This study |
| SD1 | sopD::pDM4, Cmlr | This study |
| SB2SD1 | ΔsopB sopD::pDM4, Cmlr | This study |
| SpS | spaS::pDM4, Cmlr | This study |
| S. typhimurium 12/75 | Virulent field isolate | IAH |
| S. pullorum 449/87 | Virulent field isolate | IAH |
| S. choleraisuis A57 | Virulent field isolate | IAH |
| S. enteritidis 149 | Virulent field isolate | IAH |
| S. gallinarum 287/91 | Virulent field isolate | IAH |
| Shigella sonnei PB1 | Virulent field isolate | IAH |
| Plasmids | ||
| pDM4 | oriR6K, Cmlr | 15 |
| pBScya | Ampr, pBluescript-based cya gene fusion vector | K. Schesser |
| pDelsipB1 | pDM4 vector containing the 5′ and 3′ flanking regions of sipB | This study |
| pSS1 | pDM4 vector containing XbaI-EcoRV PCR-derived insert of spaS | This study |
| pMJ600 | sopD-cya fusion plasmid, Ampr | This study |
| pMW45 | sopD positive, isolated from a pBR322-based SauIIIA library of S. dublin | This study |
a
IAH, Institute for Animal Health.