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TABLE 7.

Relative amounts of mRNA of flaB, vsp33, BH0209, BH0512, and BH0553 genes in B. hermsii DAH grown at 34°C or 24°C as determined by qRT-PCR analysis

Gene Transcript amount (ng) from growth ata:
Fold variation in mRNA amountsb
34°C 24°C
flaB 16.58 ± 0 16.58 ± 0 1.00
vsp33 18.84 ± 5.66 68.54 ± 21.74 −3.64c
BH0209 0.86 ± 0.32 0.92 ± 0.24 −1.06
BH0512 0.15 ± 0.05 0.09 ± 0.01 1.66
BH0553 2.97 ± 1.35 2.02 ± 0.46 1.47
a

Measurements of transcript amounts are based on each probe efficiency and are normalized to flaB.

b

Relative amounts of mRNA in B. hermsii DAH at 34°C and at 24°C.

c

P < 0.001.