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. 2024 Feb 16;15:1426. doi: 10.1038/s41467-024-45878-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Scatter plot depicting the change in representation of Ser3 cofilin library sequences with and without LIMK1 expression. Data points show average enrichment in glucose and galactose across three independently performed replicates. b Logo of sequences supporting growth in yeast but are selectively inhibited by LIMK1 (lower right quadrant in panel a) on the background of all sequences that support yeast growth in the absence of LIMK1 (upper and lower right quadrants in panel a). c Distribution of cofilin sequences by enrichment scores with and without LIMK1 expression according to the identity of the phosphoacceptor residue. Each distribution includes n = 8000 sequences, and values are the average from three independent screens. Box plots indicate the median (middle line), 25th and 75th percentile (box), and 10th and 90th percentile (whiskers). Source data is found in Supplementary Data 2.