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. 2024 Feb 16;14:3911. doi: 10.1038/s41598-024-54353-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental study design. Fecal samples collected from participants of two different cohorts were included in the present study. In total, 745 samples were isolated using two different DNA extraction kits: AllPrep DNA/RNA Mini Kit and QIAamp Fast DNA Stool Mini Kit (QIAGEN, Germany). After measuring DNA concentration and quality, extracted samples underwent library preparation and sequencing using the same technology (Illumina Hiseq 2000) at two different sequencing facilities. Finally, microbiome profiling and statistical analysis of sequencing data was performed.