Table 2.

Gene Ontology (GO) enrichment analysis of target genes predicted for differentially expressed miRNAs (p_adjusted < 0.05, log2FC ≥ 1.0/log2FC ≤  − 1.0) in mare endometrium samples obtained during pre-attachment period of pregnancy (day 26–28).

No	GO term ID	Term name	Term category	padjusted	Number of miRNA target genes enriched to GO term	Number of miRNA target genes enriched to particular GO term	List of miRNA target genes enriched to the particular GO term
1	GO:0015276	Ligand-gated ion channel activity	Molecular function	0.00421618620809567	787	16	CHRNA6, CHRNB3, KCNJ16, ASIC2, KCNJ13, GABRE, GABRG1, GLRA4, HTR3A, GABRB3, GLRA1, KCNJ5, ASIC3, CFTR, CHRND, CHRNA1
2	GO:0022834	Ligand-gated channel activity	molecular function	0.00421618620809567	787	16	CHRNA6, CHRNB3, KCNJ16, ASIC2, KCNJ13, GABRE, GABRG1, GLRA4, HTR3A, GABRB3, GLRA1, KCNJ5, ASIC3, CFTR, CHRND, CHRNA1
3	GO:0022824	Transmitter-gated ion channel activity	Molecular function	0.0281591760051717	787	10	CHRNA6, CHRNB3, GABRE, GABRG1, GLRA4, HTR3A, GABRB3, GLRA1, CHRND, CHRNA1
4	GO:0022835	Transmitter-gated channel activity	Molecular function	0.0281591760051717	787	10	CHRNA6, CHRNB3, GABRE, GABRG1, GLRA4, HTR3A, GABRB3, GLRA1, CHRND, CHRNA1
5	GO:0022836	Gated channel activity	Molecular function	0.0422960252917783	787	23	CHRNA6, CHRNB3, VDAC3, KCNJ16, CACNB1, ASIC2, KCNJ13, GABRE, GABRG1, GLRA4, CACNG7, KCNV2, TMEM150C, HTR3A, GABRB3, GLRA1, SNAP25, KCNJ5, KCNC4, ASIC3, CFTR, CHRND, CHRNA1
6	GO:0098960	Postsynaptic neurotransmitter receptor activity	Molecular function	0.0422960252917783	787	8	CHRNA6, CHRNB3, CHRM1, GABRG1, GABRB3, GLRA1, CHRND, CHRNA1
7	GO:0099529	Neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential	Molecular function	0.0422960252917783	787	8	CHRNA6, CHRNB3, CHRM1, GABRG1, GABRB3, GLRA1, CHRND, CHRNA1
8	GO:0099094	Ligand-gated cation channel activity	molecular function	0.0422960252917783	787	10	CHRNA6, CHRNB3, KCNJ16, ASIC2, KCNJ13, HTR3A, KCNJ5, ASIC3, CHRND, CHRNA1