FIG. 2.

SDS-PAGE analysis of LPS from IMT5155 and its mutants. The gel was loaded with whole cell lysates of strains as follows: lane 1: ladder; lane 2, wild type; lane 3, waaW mutant (M13F03); lane 4, waaL mutant (M03D10); lane 5, mutant M00G10; lane 6, waaW mutant (M12D10). The positions of the repeating subunits of the O-antigen and the core region are indicated. All mutants yielded lower LPS production compared to wild type, and waaW mutants have altered LPS core structures; waaL mutant showed differences in both O-antigens and the core region and mutant M00G10 has altered O-antigen region.