TABLE 1.
Oligonucleotide primers used in this study and results of PCRs to detect genes of pathogenicity island
| Primer | Sequence (5′-3′) | Presence of genea |
|---|---|---|
| P2 | TACCTACAACCTCAAGCT | |
| P4 | TACCCATTCTAACCAAGC | |
| P6 | CCTAGGCGGCCAGATCTGAT | |
| P7 | GCACTTGTGTATAAGAGTCAG | |
| P9 | CGCAGGGCTTTATTGATTC | |
| Arbi1 | GGCCACGCGTCGACTAGTAC(N)10 GATAT | |
| Arbi2 | GGCCACGCGTCGACTAGTAC | |
| Arbi3 | GGCCACGCGTCGACTAGTAC(N)10 TGACG | |
| Arbi4 | GGCCACGCGTCGACTAGTAC(N)10 ACGCC | |
| Arbi5 | GGCCACGCGTCGACTAGTAC(N)10 TACNG | |
| P19 | ATTCAACGGGAAACGTCTTG | |
| P20 | ACTGAATCCGGTGAGAATGG | |
| PapG-F | TTTGCGAGTGGAGTGTATTT | |
| PapG-R | TACCTAACCCAACCGAAAAT | |
| PapC-F | TGATATCACGCAGTCAGTAGC | |
| PapC-R | CCGGCCATATTCACATAA | |
| R14-15-F | GCCAGTGACACATACTGAGAGC | + |
| R14-15-R | CAGATGTACAGTGGCGCG | |
| R2 plus R3-F | GCTGTCAGAATATTTCGCTCG | + |
| R2 plus R3-R | AGTCCTGTCACGCTGAACG | |
| R1 plus R2-F | AGCCTTTCTGTTTTGAGCAT | + |
| R1 plus R2-R | TCGCTACTATTGATTCTTGC | |
| R1 plus f447-F | CCGCAAGAATCAATAGTAGC | + |
| R1 plus f447-R | CTGGCGAGAAGGGGATAATG |
a
PCR elaborated for detection of metV genomic island.